Development of gene drives and a method to block them in Anopheles gambiae.

Table of Contents below.
Dr. Taxiarchi describes the creation and laboratory testing of a first-generation 'anti-drive' system based on CRISPR/Cas9 inhibitory proteins in the human malaria mosquito Anopheles gambiae.

TABLE OF CONTENTS
00:00 Speaker Introduction (David O'Brochta, Ph.D.)
1:06 Background: Why Anophlese gambiae?
2:13 Number of mosquito species, 3 major vectors in Africa
2:32 Malaria transmission genetically determined
2:40 Genetic modification to affect transmission
3:10 Challenge – spread modification
4:05 Gene drive genetics
4:35 Homing drive explanation –
5:50 Population suppression or modification
6:35 Population suppression – female fitness
7:44 Sex determination background
8:26 Doublesex of Anophese gambiae
9:18 Disruption of exon 5 of doublesex
10:13 Homozygous female do not bite and are sterile
11:26 Gene drive design
12:42 Genetic evidence for drive
13:17 Cage experimental design
14:58 Cage experimental data
16:30 No resistance evolved
17:17 Inhibiting the spread of drive: anti-Drive Mosquito
18:08 AntiCRISPR proteins (Acrs)
18:50 AcrIIA4 description
19:30 AcrIIA4 blocks DNA binding and cutting
20:27 Anti Drive Construct
22:03 Anti-Drive effect in A. gambiae-test crosses
23:50 Anti-Drive effect in A. gambiae-cage experimental design
25:49 Cage experimental data
27:42 Stochastic population modelling
28:28 Fitness cost of Anti Drive
30:24 Population Modeling Fitness costs
32.55 Acknowledgements
33:37 Questions and Answers

gene drivegenetic biocontrolpublic health